Metabolism of propionic acid in animal tissues. V. Purification and properties of propionyl carboxylase.
نویسندگان
چکیده
action 2a, might react according to Reaction 3, in the presence of propionyl-CoA carboxylase, to form methylmalonyl-CoA. In order to clarify the mechanism of propionyl-CoA carboxylation further purification of the ‘Yluorokinase” and carboxylase systems was undertaken. L’F1uorokinase” was crystallized from rabbit muscle and, as already reported (4), appears to be identical with pyruvic kinase, the enzyme which catalyzes the reversible transfer of phosphate from ATP to pyruvate. The present
منابع مشابه
Metabolism of Propionic Acid in Animal Tissues
The conversion of propionyl coenzyme A to succinyl-CoA involves carboxylation to methylmalonyl-Cob (a), isomerization of methyhnalonyECoA (a) to its enantiomorph methyhnalonylCoA (b), and isomerization of the latter to succinyl-CoA (3). Since methylmalonyl-CoA (a) has recently been shown to have the D configuration (4, 5), the reversible reaction catalyzed by methylmalonyl-CoA racemase will hen...
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The conversion of propionyl coenzyme A to succinyl-CoA involves carboxylation to methylmalonyl-Cob (a), isomerization of methyhnalonyECoA (a) to its enantiomorph methyhnalonylCoA (b), and isomerization of the latter to succinyl-CoA (3). Since methylmalonyl-CoA (a) has recently been shown to have the D configuration (4, 5), the reversible reaction catalyzed by methylmalonyl-CoA racemase will hen...
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The enzymes of fatty acid synthesis from liver and brain in normal and Blz-deprived rats were studied. Both total and specific activities of fatty acid synthetase and acetyl coenzyme A carboxylase were 2to 5-fold greater in Blz deprivation than in the normal state. The presence of excess activators in the BIp-deprived rat or an excess inhibitor in the normal rat was not found by serial admixtur...
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A 4-month-old girl presented with myoclonic seizures and an electroencephalogram showing hypsarrhythmia. Hyperglycinuria and a cerebrospinal fluid to plasma glycine ratio of 0.2 suggested the diagnosis of non-ketotic hyperglycinaemia. Propionic acid and methyl citric acid were present in the urine, and propionyl coenzyme A carboxylase was deficient in leucocytes and fibroblasts. The ketotic and...
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Mycobacterium tuberculosis H37Ra and M. bovis BCG produce multiple methyl-branched fatty acids called mycocerosic acids, presumably from methylmalonyl coenzyme A (CoA). An acyl-CoA carboxylase was isolated from these organisms at a 30 to 50%fo yield by a purification procedure involving ammonium sulfate fractionation, gel ifitration, and affinity chromatography with a monomeric avidin-Sepharose...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 234 6 شماره
صفحات -
تاریخ انتشار 1959